Wrapup

Overview

Teaching: 10 min
Exercises: 0 min

Questions

• Do my findings match the published data?

Objectives

• Compare the findings with predictions by other prediction programs

Exercise

Compare your lists to the pre-computed lists at PSORTdb for E.coli K-12 and S.aureus N315. Do you find proteins in common? Do you find differences? Why? Discuss with your neighbor.

We predicted if proteins are secreted based on the presence of signal peptides. Prediction can be different, depending on the method used for prediction, but also based on annotation differences, differences in ORF calling etc. In order to confirm the cellular location of these proteins, laboratory experiments are necessary.

Wrapup

Summary

What have you done today? Write your name and a short summary of about 5 sentences on the collaborative document.

Key Points

• Computational predictions need to be validated by laboratory experiments